In the eastern half of the country (sites SoUt32-SoTi69), 60 Pocillopora spp. colonies were sampled (SoPd66-125). Unfortunately, I temporarily lost samples SoPd118-125 and so nucleic acids were never extracted from them. I have since found these samples, so I hope to analyze them in the future.

UTUPUA (“Ut;” 2015-11-10 to 11-13) For high-resolution habitat maps, see https://maps.lof.org/lof.

2014-11-10 (SoUt32-34)

SoUt32: sampled three Pocillopora spp. (SoPd66-68) & three Seriatopora spp. colonies (SoSe99-101)

SoPd66

This presumed P. acuta colony was not analyzed due to having used these damned Direct-zol columns, which get clogged with coral skeleton. As such, I have no RNA or DNA for this biopsy. Other data can be found here:

SoPd67

This P. acuta colony was genotyped though not analyzed in full on account of a failed RNA extraction (due to using Direct-zol columns). Other data can be found here:

supplemental data (JMP)

supplemental data (Excel)

SoPd68

This P. brevicornis colony was genotyped though not analyzed in full on account of it being a non-target species. Other data can be found here:

supplemental data (JMP)

supplemental data (Excel)

SoUt33: night dive site. Tagged eight Pocillopora sp. colonies (SoPd69-76) and sampled during both day and night. Despite its P. acuta-like appearance, SoPd72 was genotyped as P. brevicornis. Did not sample Seriatopora spp.

SoPd69

This P. acuta colony was sampled in both day and night, though the night sample was lost, and the RNA extraction for the day sample failed. Other data can be found here.

SoPd70

This P. acuta colony was tagged, sampled in both day and night, genotyped, and analyzed in full. The colony demonstrated aberrant behavior at night only (high RNA/DNA ratio) and was therefore considered an outlier:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd71-outlier

This P. acuta colony was tagged, sampled in both day and night, genotyped, analyzed in full, and found to be displaying aberrant behavior during both day and not (abnormally high GFP-like chromoprotein mRNA expression in the coral host at both times). It was therefore flagged as an outlier:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd72-P. brevicornis

Although it looks like P. acuta, this colony was genotyped as P. brevicornis, and was consequently excluded from the data analysis since the sample size was only eight. Other data can be found here:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd73

Lost whole-colony image. This P. acuta colony was strange in that clade A (Symbiodinium) dinoflagellates were only detected in the daytime biopsy; the night time biopsy’s dinoflagellate community was exclusively Cladocopium (clade C). Otherwise, the colony’s behavior was statistically normal:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd74

This P. acuta was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd75

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd76

This P. acuta colony was tagged, sampled in both day and night, genotyped, yet the night biopsy was lost, and RNAs were not extracted from the daytime biopsy. Other data can be found here: supplemental data (JMP)

supplemental data (Excel)

SoUt34: did not dive on account of setting up for night dive.

2014-11-11 (SoUt35-37)

SoUt35: Only four Seriatopora spp. colonies (SoSe102-105) were sampled at this site due to ripping currents. No Pocillopora spp. were sampled.

SoUt36: Three Pocillopora spp. (SoPd77-78=P. acuta and SoPd79=P. brevicornis) and two Seriatopora spp. (SoSe106-107) were sampled.

SoPd77

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd78

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd79

This P. brevicornis colony was genotyped and analyzed in full though not included in the outlier analysis since it was a non-target species. Other data can be found here:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoUt37: Sampled two P. acuta (SoPd80-81) and three Seriatopora spp. (SoSe108-110) colonies.

SoPd80

This P. acuta colony was genotyped though not analyzed in full (despite having extracted both RNA and DNA). Other data can be found here:

supplemental data (JMP)

supplemental data (Excel)

SoPd81

This P. acuta colony was genotyped though not analyzed in full. Other data can be found here:

supplemental data (JMP)

supplemental data (Excel)

2014-11-12 (two dives only)

SoUt38: Neither P. acuta/P. damicornis nor Seriatopora spp. were observed at this site.

SoUt39: Two P. acuta (SoPd82-83) and four Seriatopora spp. (SoSe111-115) colonies were sampled from this site.

SoPd82

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd83

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)
supplemental data (Excel)

SoPd83

2014-11-13 (SoUt40-42)

SoUt40: Neither P. acuta/P. damicornis nor Seriatopora spp. were observed at this site.

SoUt41: Four P. acuta (SoPd 84-87) and eight Seriatopora spp. (SoSe116-123) were sampled from this site.

SoPd84

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd85

Cannot locate full colony image (macro only)

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd86

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd87

This P. acuta colony was genotyped but not analyzed in full; other data can be found here:

supplemental data (JMP)

supplemental data (Excel)

SoUt42: did not dive.

VANIKORO (“Va;” 2015-11-14 to 10-16) For high-resolution habitat maps, see https://maps.lof.org/lof.

2014-11-14 (SoVa43-45)

SoV43: sampled two P. acuta (SoPd88-89) and five Seriatopora spp. (SoSe124-128) colonies.

SoPd88

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd89

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoVa44: sampled two P. acuta (SoPd90-91) and four Seriatopora spp. (SoSe129-132) colonies.

SoPd90

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd91

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)
supplemental data (Excel)

SoVa45: sampled three P. acuta (SoPd92-94) and two Seriatopora spp. (SoSe133-134) colonies.

SoPd92

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd93

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd94

This P. acuta colony was genotyped and analyzed in full (though I forgot to note the extraction conditions for some reason):

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

2014-11-15 (SoVa46-48)

SoVa46: Seriatopora spp. were not observed at this site. Two Pocillopora spp. colonies (SoPd95 and 96) were sampled (genotyped as P. brevicornis and P. damicornis, respectively).

SoPd95

This P. brevicornis colony was genotyped and analyzed in full; however, since it was a non-target species, it was not included in the outlier analysis.

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd96

This P. damicornis colony was genotyped and analyzed in full; however, it was excluded from the outlier analysis given that only three P. damicornis colonies were sampled from the Solomon Islands.

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoVa47: sampled three P. acuta (SoPd97-99) and eight Seriatopora spp. (SoSe135-142) colonies.

SoPd97

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd98

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd99

This P. acuta colony was genotyped and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoVa48: sampled one Pocillopora sp. (SoPd100; not genotyped) and five Seriatopora spp. (SoSe143-147) colonies.

SoPd100

Nucleic acids were not extracted from this sample. Other data can be found here.

2014-11-15: did not dive at sites SoVa49-51.

Instead returned to SoVa47 for two additional dives since there were so many Pocillopora spp. colonies there displaying aberrant coloration (SoPd101-104). Only sampled one Seriatopora sp. colony (SoSe148) during the latter of these two dives (SoVa47-round 3).

SoPd101

Both normally and abnormally pigmented sections of this P. acuta colony were sampled. Neither portion was found to be displaying aberrant behavior, though:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd102-normal vs. abnormal

Both normally and abnormally pigmented sections of this P. acuta colony were sampled, and, strangely enough, the normally pigmented biopsy had such a high dinoflagellate density as to warrant its labeling as an outlier:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd103

Unlike the previous two colonies, this one (P. acuta) was 100% abnormally pigmented (purple), so the two biopsies are essentially redundant (and the data can likely be pooled):

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd104: normal and abnormal

Both normally and abnormally pigmented biopsies were taken from this P. acuta colony; neither biopsy, though, was displaying aberrant behavior:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

REEF ISLANDS (“Ri;” 2014-11-17 to 11-21) For high-resolution habitat maps, see https://maps.lof.org/lof.

2014-11-17 (SoRi52-54)

SoRi52: sampled one P. acuta (SoPd105) and 10 Seriatopora spp. (SoSe149-158) colonies.

SoPd105 normal vs .abnormal

Both normally and abnormally pigmented biopsies were removed from this P. acuta colony, though neither biopsy was found to be displaying aberrant cellular behavior:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoRi53: night dive site. Tagged and sampled five P. acuta colonies (SoPd106-110) in both the day and night. Also sampled four Seriatopora spp. colonies (SoSe159-162).

SoPd106

This P. acuta colony was tagged, sampled in both light and dark, and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd107

This P. acuta colony was tagged, sampled in both day and night, genotyped, and analyzed in full; the night time biopsy was found to be displaying aberrant behavior (predominantly due to an abnormally high RNA/DNA ratio):

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd108 light-dark

This P. acuta colony was tagged, sampled in both day and night, genotyped, and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd109 light-dark

This P. acuta colony was tagged, sampled in both day and night, genotyped, and analyzed in full:

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd110

SoPd110 light and dark

This P. acuta colony was tagged, sampled in both day and night, genotyped, and analyzed in full. Interestingly, the dinoflagellate assemblage differed over time; clade D cells were only detected in the daytime biopsy, not in the night time one. This could be due to having sampled from a different part of the colony (not related to time).

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoRi54: did not dive on account of setting up for night dive.

Reef Islands day 2: 2014-11-18 (SoRi55-57)

SoRi55: Only sampled Seriatopora spp. (SoSe163-169) at this site.

SoRi56: sampled one P. acuta (SoPd111) and two Seriatopora spp. (SoSe170-171) colonies.

SoPd111

This P. acuta colony was genotyped though not analyzed in full; other data can be found here:

supplemental data (JMP)

supplemental data (Excel)

SoRi57: only sampled P. acuta (SoPd112-114) at this site.

SoPd112

This presumed P. acuta colony was not analyzed in full due to a failed DNA extraction. Other data can be found here.

SoPd113-outlier

Both normally pigmented and bleached portions of this P. acuta colony were sampled, and the healthy biopsy was characterized by aberrant GFP-like chromoprotein gene expression; the sample was consequently delineated as an outlier.

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd114

Both normally pigmented and bleached/dying portions of this P. acuta colony were sampled, and neither biopsy, curiously, demonstrated aberrant cellular behavior.

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd114

Reef Islands day 3: 2014-11-19 (SoRi58-60)

SoRi58: sampled three P. acuta (SoPd115-117) and five Seriatopora spp. (SoSe172-176) colonies.

SoPd115

Both normally pigmented and bleached portions of this P. acuta colony were sampled. Unfortunately, the RNA spike assay performed poorly with one of the two biopsies; it was thus removed from the dataset and not considered in the statistical analysis. I am uploading data, but please realize that the aberrant values are likely methodological in nature (and not necessarily because the coral itself was behaving strangely).

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoPd116

This P. acuta colony was genotyped but not analyzed in full; other data can be found here:

supplemental data (JMP)

supplemental data (Excel)

SoPd117 normal vs. abnormal

Both normally and abnormally pigmented (likely dying) tissues were sampled from this P. acuta colony; strangely enough, neither biopsy was displaying aberrant cellular behavior.

JMP

Excel

supplemental data (JMP)

supplemental data (Excel)

SoRi59: sampled one Pocillopora sp. (SoPd118; not processed) and six Seriatopora spp. (SoSe177-182) colonies.

SoPd118-not yet analyzed

Both normally and abnormally pigmented biopsies were sampled from this presumed P. acuta colony. I hope to analyze them in the future. Other data can be found here.

SoRi60: sampled three Pocillopora spp. colonies (SoPd119-121) only. These samples got mixed up in the Palau samples box and so were never extracted, but I hope to get to them in the future. For these samples, as well as SoPd101-104, SoPd113-115, and SoPd117-119, I sampled both a normally pigmented biopsy and an abnormally pigmented one (bleached, in some cases) from the same colony to look at intra-colony differences.

SoPd119 normal vs. abnormal

Both normally and abnormally pigmented biopsies were removed from this presumed P. acuta colony, though neither was processed due to having temporarily misplaced the tubes. I aim to get back to these in the future. Otherwise, supplemental data about the colony can be found here.

SoPd120 normal vs. abnormal

Nucleic acids have not yet been extracted from the normally and abnormally pigmented biopsies taken from this sample. Other data can be found here.

SoPd121

Sampled both normally and abnormally pigmented sections of this presumed P. acuta colony. Nucleic acids have not yet been extracted. Other data can be found here.

Reef Islands day 4: 2014-11-20 (SoRi61-63 and SoRi56-round 2)

SoRi61-62: did not dive. Instead, I returned to site SoRi56 to sample more Seriatopora spp. colonies (n=7: SoSe183-189).

SoRi63: sampled one Pocillopora sp. (SoPd122; never processed) and two Seriatopora spp. (SoSe190-191) colonies.

SoPd122

Nucleic acids have not yet been extracted from this presumed P. acuta colony. Other data can be found here.

Reef Islands day 5: 2014-11-21 (SoRi64-66)

SoRi64: neither P. acuta nor P. damicornis present; only sampled Seriatopora spp. (SoSe192-193).

SoRi65: only sampled one Seriatopora sp. colony (SoSe194) on account of strong currents.

SoRi66: sampled two Pocillopora spp. (SoPd123-124; neither processed) and six Seriatopora spp. (SoSe195-200) colonies.

SoPd123

Both abnormally and normally pigmented sections of this presumed P. acuta colony were sampled. I hope to analyze the samples in the future; other data can be found here.

SoPd124

Nucleic acids have not yet been extracted from this presumed P. acuta colony; other data can be found here.

TINAKULA (“Ti;” 2014-11-22) For high-resolution habitat maps, see https://maps.lof.org/lof. Seriatopora spp. were not present at Tinakula Volcano.

SoTi67: Only one Pocillopora sp. observed and sampled (SoPd125; not processed).

SoPd125

Nucleic acids have not yet been extracted from this presumed P. acuta colony. Other data can be found here.

SoTi68: Neither P. acuta/P. damicornis nor Seriatopora spp. observed.

SoTi69: Neither P. acuta/P. damicornis nor Seriatopora spp. observed.