Interactive protocol browser

Mayfield et al. Fig. 2.jpg

Protocols, Reagents & Analysis Methods

Molecular, protein, physiological-assay, histology, bioinformatics, and statistical-methodology resources used across our NSF/NOAA/LOF/post-doc projects, spanning corals, anemones, sponges, algal symbionts, and seagrass. Search by name, target species, gene/analyte, or keyword; filter by category and/or by type below -- "Our own protocol" is a real step-by-step method we wrote or adapted, "Manufacturer/kit info" is the vendor's own product documentation (still useful, just not our method). Click a protocol name to download the file, the “Manufacturer info ↓” tag to download that vendor’s protocol/brochure, or the Manufacturer name to visit that company’s site.

All RNA/DNA extraction & QC qPCR / cDNA Protein / Western blot Physiological assays Histology Lipids & metabolomics Multi-omics extraction Bioinformatics pipelines Statistics JMP Reference publications Reef survey methods
All types Our own protocol Manufacturer/kit info Tool / template Publication
Published + unpublished Published only Unpublished / in prep only

Descriptions marked in orange italics are our best guess from the filename and should be double-checked. Some entries are decades old (see Year) and kept for historical reference -- check with us before using as-is on a new species. In the Rep. publication column, blue-gray citations are our own papers demonstrating real use; tan citations are the field's standard method/tool paper (not necessarily ours). Want to learn more about our bioinformatics pipelines? Visit the Multi-’Omics Server. Questions? Email anderson@coralreefdiagnostics.com.

Molecular Biology: RNA, DNA, reverse transcription, exogenous spikes, & real-time PCR

NMMBA's coral buoyant weighing station

NMMBA's coral buoyant weighing station

Physiology

 32. buoyant weighing

 33. coral density calculations

 34. chlorophyll measurements

 35. Symbiodiniaceae density      36. surface area

I envision using 3D scanning tools for all future surface area measurements.

Blocks for coral surface area standard curve generation

Blocks for coral surface area standard curve generation

Dr. Coral 🪸
Your Coral Reef Research Assistant
Checking...
🪸 Hello! I'm Dr. Coral. I can help you with: 📚 Research questions (45,000+ scientific papers) 🌍 Finding photos and data from coral reefs from across the globe 🌱 Coral reef conservation and restoration planning What would you like to know?