One day early in the trip (2015-1-10) and two later on (2015-2-8 to 2-9) were spent surveying and sampling the bizarre, high-pCO2 reefs of stagnant, isolated, and hidden Nikko Bay. No Pocillopora damicornis colonies were sampled; all were later genotyped as Pocillopora acuta (using the Johnston et al. 2018 Peer J RFLP assay courtesy of Dr. Ben Wainwright). Since RFLPs were used for genotyping, rather than PCR+sequencing as for prior research cruises, no links to NCBI pages have been provided.

High-resolution habitat maps will be placed on the Living Oceans Foundation’s excellent mapping  site  in late 2019.

High-resolution habitat maps will be placed on the Living Oceans Foundation’s excellent mapping site in late 2019.

PaNi01 (2015-1-10) lagoonal fringing reef

PaNi01 sampled pocilloporid colonies (n=3). I will overlay the molecular physiological data files onto the images (as per the other “Global Reef Expedition” samples) in the coming months of 2019.

PaNi01 sampled Seriatopora hystrix colony (n=1)

Our return visit at the end of the mission (2015-2-8 to 2-9): PaNi85 (no pocilloporids sampled and no seriatoporids observed; please note that this was virtually the same site as PaNi01)

PaNi86 (2015-2-9): three pocilloporids were sampled (see below.) though no seriatoporids were observed at this lagoonal fringing reef.

PaNi86 sampled P. acuta colonies (n=3). Please note that, in general, I maintained the “Pd” sample code since, at that time, we were not yet confident in our ability to distinguish P. damicornis from P. acuta.

PaNi87 (2015-2-9) P. acuta was common here but not sampled. Instead, I mainly sampled seriatoporid colonies (see images posted below the site pictures.) at this lagoonal fringing reef.

Seven seriatoporid colonies were sampled from PaNi87 (PaSe211-217), though images were not taken of the final one (PaSe217).