In April 2013, the Khaled bin Sultan Living Oceans Foundation visited 30 reef sites across five major islands/atolls within the Austral Islands of French Polynesia. It should be noted that samples from the Cook Islands are typically found within the spreadsheets referenced (and linked) below, as the Cook Islands were visited immediately after the Austral Islands. Habitat shots of the majority of the reefs visited, as well as photos of all sampled colonies, can be found (in order of data surveyed) below. I apologize for the poor quality of the images, as it was my first time to use an underwater camera; I did not even know about white balancing, for instance, until midway through the cruise!
On the pages for the islands visited (i.e., Raivavae, Tubuai, Rurutu, Rimatara, and Maria [NOT Moorea]), images of the sampled colonies have been unloaded. For those biopsies analyzed in detail, hyperlinks have been overlaid on the colony images to either take interested individuals to websites with more information (e.g., NCBI for DNA sequence information) or download pertinent data files (JMP and/or Excel format). If you are interested in 1) histology cassettes, 2) RNAs, 3) DNAs, or 4) proteins from these samples, please email me (email@example.com), and we will try to figure out how to make it happen. The DNAs and histology cassettes (only 20-30 samples of the 122 total colonies samples) will be easy to mail, though the RNAs and proteins may require a dry shipper.
2. For a summary of the environmental data, as well as information on the genotypes on the 60 pocilloporid corals sampled, please check out the following manuscript: Mayfield et al. (2015, Platax; click here). It should be noted that corals were collected and exported under a CITES permit, and nucleic acid extractions were carried out as described here.
3. For the GPS coordinates of the study sites, as well as site temperatures and salinities, please click here.
4. To see the coral cover data at different depth ranges for each of the sites, please click here.
5. To see the environmental and physiological data associated with each of the 60 samples, please click here (first worksheet).
6. To see the molecular data for these same samples, please check out this manuscript (Mayfield et al. 2016, Platax). The gene expression data, which are the main focus of this manuscript, can be found here.
7. For a summary of all NCBI accession numbers for the genotyped colonies, please click here.
8. For a list of all pocilloporid corals sampled as part of the Living Ocean Foundation's Global Reef Expedition, please click here.
For those of you with JMP (a truly excellent statistical package), here are the JMP-formatted data files:
For the third manuscript (in review in Journal of Sea Research), I used this dataset.
Interactive PCA plot. Hover over the icons to get more information (their images are not loading properly, an issue I hope to resolve soon).
I have also posted the dataset on JMP’s website to try to get a fresh perspective on it: https://community.jmp.com/t5/JMP-Sample-Data/Reef-coral-data-from-the-Austral-Islands-French-Polynesia-and/ta-p/61858
Here are the fixed and decalcified coral tissues prior to embedding in paraffin (hosted on my One Drive account):
Papeete-I would really like to spend more time here in the future, having passed through so often.