In June 2013, the Living Ocean Foundation's research vessel the M.Y. Golden Shadow traversed Fiji's Lau Archipelago (11 islands listed in the map above). For details please check out this project report. Lau has been plagued by over-harvesting of sea cucumbers, and so there was an emphasis on assessing sea cucumber diversity and abundance; see this report by Stacey Jupiter for details. Also, if you would like the photos of the reef habitats and sampled corals in a consolidated Aperture/iPhoto library, please email me (email@example.com) or download it from my GoogleDrive account. For the NCBI accession numbers of the Pocillopora spp. colonies analyzed, please consult this spreadsheet. Please note that RNAs, DNAs, and proteins were not extracted from all 153 sampled colonies. For the molecular-scale data for a subset of these colonies, please click here. Otherwise, links to data files have been overlaid onto the colony images posted on the respective pages for each island visited.
In this manuscript, I used the Mahalanobis distance (amongst other means) to assign a level of aberrancy to each sample. This essentially ranks the colonies sampled in terms of how "weird" they are; although we can't say it for sure at the moment, statistically oddly behaving colonies may be those that are ultimately found to also be stressed or of compromised resilience. I have now taken another look at this dataset to see which environmental parameters were most important in driving physiological variation between the sampled colonies. As those who work on corals know, there is a ridiculous amount of inter-colony variation in physiology, and we don't have a good handle on why this is the case. I had hypothesized previously that it was due to differences in light, but my data do not currently support this notion. It instead appears to be driven more by genetic variation in the host, a wholly unsurprising finding discussed in this second manuscript, which also features a variety of multivariate statistical approaches.
For GPS coordinates of the 70 site surveyed, please click here. For information on the reefs (exposure, type, zone, and coral cover), please click here. Other environmental data can be found in this JMP file. Structural details of the reefs and remote sensing data can be found in Saul and Purkis (2015). Ultimately, all maps produced and underwater drop-camera footage taken will be posted on the following website (currently undergoing maintenance): http://maps.lof.org/lof. If you want to create the maps yourself, here are the shape files: name X-Y
2. Mitochondrial open reading frame (mORF) sequences of the sampled colonies. Alternatively, hyperlinks to the NCBI pages hosting the mORF sequences have been overlaid on the images of the sampled colonies (found on the respective pages for the islands visited).
3. For details on the 11 outliers only, click here.
4. For data (as JMP files) organized in all different ways, download this folder from my OneDrive page.
5. For an interactive PCA plot, please go here.
Below I have posted some shots of our land excursions (namely to drink kava with friendly locals).